Detection and Genotyping of Astroviruses by RT-PCR and Sequencing
نویسندگان
چکیده
منابع مشابه
Detection and Genotyping of Hepatitis D Virus from HBsAg Positive Patients in Iran Using RT-PCR
Hepatitis Delta virus (HDV) is a degenerate RNA virus or virusoid and a satellite of Hepatitis B virus (HBV). Three distinct genotypes are described for HDV; genotype I is distributed worldwide but other genotypes appear to be more restricted geographically. In the present study, an RT-nested PCR method was set up to detect delta infection from serum samples. Moreover, the target amplified sequ...
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1320 blood samples were collected from herds showing clinical signs of pestiviral infections. 39 samples were positive in Ag-ELISA assay. All these 39 samples in addition to 5 cytopathic strains were cultured in MD-BK cell line and presence of pestiviral antigens was confirmed by direct-immunofluorescent test. 27 out of 44 BVDV suspected isolates were detected by RT-PCR using a primer set. Diff...
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Rapid antigen and antibody, serological tests, and RT ‑ PCR-based molecular methods are widely used for the detection of microorganisms worldwide. Objectives: This study aimed by Isothermal nucleic acid amplification techniques to detect the covid-19. Methods: In this study, 200 samples of nasopharynx and oropharynx were collected from Jamaran Heart Hospital in Tehran. Covid -19 was exami...
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OBJECTIVE To analyze the sequence difference between human A, B, and O alleles and establish the method of ABO genotyping by PCR direct sequencing. METHODS PCR-direct sequencing technique was used to analyze two regions of cDNA from A transferase gene, 233-433 and 660-788. RESULTS Two nucleotide substitutions at 258th and 297th were found in 233-433 region, and a nucleotide substitution at ...
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Human papillomavirus (HPV) infection is the most common sexually transmitted disease worldwide and there is a strong link between certain high-risk viral types and cervical carcinogenesis. Although there are several typing methods, it is still unclear which test is the best. This study compared the effectiveness of type-specific PCR (TS-PCR) and sequencing, with a focus on their clinical applic...
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ژورنال
عنوان ژورنال: Journal of the Japanese Association for Infectious Diseases
سال: 1996
ISSN: 1884-569X,0387-5911
DOI: 10.11150/kansenshogakuzasshi1970.70.1220